51. (Journal Article): Enriched Human Pancreatic Ductal Cultures Obtained from Selective Death of Acinar Cells Express Pancreatic and Duodenal Homeobox Gene-1 Age-Dependently

Street CN, Lakey JR, Rajotte RV, Shapiro AM, Kieffer TJ, Lyon JG, Kin T, Korbutt GS
 
ABSTRACT: Adult pancreatic ductal cells are believed to be islet precursors. Our aim was to obtain an enriched human ductal cell population in defined culture conditions, and to characterize...

52. (Journal Article): In vitro study of elements in herbal remedies.

Szentmihalyi K,, Hajdu M, Fodor J, Kotai L, Blazovics A, Somogyi A, Then M.
 
ABSTRACT: Decreased glucose tolerance is a first sign of diabetes mellitus and therefore rigorous control must be taken in carbohydrate and lipid metabolisms. Herbal remedies (lyophilized ex...

53. (Journal Article): XIAP overexpression in human islets prevents early posttransplant apoptosis and reduces the islet mass needed to treat diabetes

Emamaullee JA, Rajotte RV, Liston P, Korneluk RG, Lakey JR, Shapiro AM, Elliott JF
 
ABSTRACT: The Edmonton Protocol for treatment of type 1 diabetes requires islets from two or more donors to achieve euglycemia in a single recipient, primarily because soon after portal infu...

54. (Journal Article): Restoration of self-tolerance is a feasible approach to control ongoing beta-cell specific autoreactivity: its relevance for treatment in established diabetes and islet transplantation

Chatenoud L
 
ABSTRACT: Major progress has been made over the last 10 years in understanding the immune mechanisms underlying autoimmune Type I (insulin-dependent) diabetes mellitus. It seems that immunot...

55. (Journal Article): Influence of cell number on the characteristics and synchrony of Ca2+ oscillations in clusters of mouse pancreatic islet cells.

Jonkers FC, Jonas JC, Gilon P, Henquin JC
 
ABSTRACT: 1. The cytoplasmic Ca2+ concentration ([Ca2+]i) was measured in single cells and cell clusters of different sizes prepared from mouse pancreatic islets. 2. During stimulation with ...

56. (Journal Article): Genetically selected cardiomyocytes from differentiating embryonic stem cells form stable intracardiac grafts.

Klug MG, Soonpaa MH, Koh GY, Field LJ
 
ABSTRACT: This study describes a simple approach to generate relatively pure cultures of cardiomyocytes from differentiating murine embryonic stem (ES) cells. A fusion gene consisting of the...

57. (Journal Article): Insulin-secreting cells derived from embryonic stem cells normalize glycemia in streptozotocin-induced diabetic mice.

Soria B, Roche E, Berna G, Leon-Quinto T, Reig JA, Martin F
 
ABSTRACT: Embryonic stem (ES) cells display the ability to differentiate in vitro into a variety of cell lineages. Using a cell-trapping system, we have obtained an insulin-secreting cell cl...

58. (Journal Article): Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.

Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A
 
ABSTRACT: We describe the derivation of pluripotent embryonic stem (ES) cells from human blastocysts. Two diploid ES cell lines have been cultivated in vitro for extended periods while maint...

59. (Journal Article): Derivation of pluripotent stem cells from cultured human primordial germ cells.

Shamblott MJ, Axelman J, Wang S, Bugg EM, Littlefield JW, Donovan PJ, Blumenthal PD, Huggins GR, Gearhart JD
 
ABSTRACT: Human pluripotent stem cells would be invaluable for in vitro studies of aspects of human embryogenesis. With the goal of establishing pluripotent stem cell lines, gonadal ridges a...

60. (Journal Article): Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro.

Shamblott MJ, Axelman J, Littlefield JW, Blumenthal PD, Huggins GR, Cui Y, Cheng L, Gearhart JD
 
ABSTRACT: Human pluripotent stem cells (hPSCs) have been derived from the inner cell mass cells of blastocysts (embryonic stem cells) and primordial germ cells of the developing gonadal ridg...