(Journal Article): Isolation and characterization of a cell line from the epithelial cells of the human fetal pancreas.
 
Wang S, Beattie GM, Mally MI, Cirulli V, Itkin-Ansari P, Lopez AD, Hayek A, Levine F (Department of Pediatrics, UCSD School of Medicine, La Jolla, CA 92093-0634, USA.)
 
IN: Cell Transplant 1997; 6(1):59-67
Impact Factor(s) of Cell Transplant: 2.327 (2003), 2.42 (2002), 2.19 (2001)

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ABSTRACT: Pancreatic cell lines are useful for basic studies of pancreatic biology and for possible application to cell transplantation therapies for diabetes. A retroviral vector expressing simian virus 40 (SV40) T antigen and H-rasval12 was used to infect a monolayer culture of epithelial cells from an 18-wk human fetal pancreas. Infected cells gave rise to a clonal epithelial cell line, designated TRM-1. This cell line expresses epithelial markers as well as gult2 and small amounts of insulin and glucagon. TRM-1 is the first cell line to be generated from the human fetal pancreas and also the first cell line derived directly from the fetal pancreas of any species. The approach that we have used to develop TRM-1 should be applicable to isolating cell lines from other stages of human pancreatic development.

TYPE OF PUBLICATION: Original article

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• Hao E, Tyrberg B, Itkin-Ansari P, Lakey JR, Geron I, Monosov EZ, Barcova M, Mercola M, Levine F. Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas. Nat Med 2006. 12: 310-316.
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