|
|
(Journal Article): Adult human pancreatic duct and islet cells exhibit similarities in expression and differences in phosphorylation and complex formation of the homeodomain protein IPF-1.
Heimberg H, Bouwens L, Heremans Y, Van den Casteele M, Lefebvre V, Pipeleers D (Diabetes Research Center, Vrije Universiteit Brussel, Belgium.,
hheimber@vub.vub.ac.be
)
IN:
Diabetes
2000; 49(4):571-579
Impact Factor(s) of Diabetes: 8.848 (2004), 8.298 (2003), 8.256 (2002), 7.7 (2001)
Fulltext:
HTML
PDF
ABSTRACT: The homeodomain transcription factor encoded by the pancreatic and duodenal homeobox gene-1 (Ipf-1) is essential for pancreatic ontogenesis. Whether Ipf-1 is also involved in the neogenesis of beta-cells in the adult pancreas is unknown. We examined whether Ipf-1 is expressed in adult human pancreatic ducts, which are thought to generate new beta-cells. In tissue sections, virtually all duct cells were immunopositive for Ipf-1, as were the islet beta-cells but not the acinar cells. After isolation and culture, both duct and islet cell preparations contained the Ipf-1 immunoreactive proteins p42 and p45 (42 and 45 kDa, respectively) in similar proportions, but the expression levels were twofold lower in duct cells. After 4 h of labeling, the endocrine cells exhibited a sevenfold higher phosphorylation of p42 than the duct cells, whereas p45 was phosphorylated only in endocrine cells. Homeobox binding transcription factor complexes with Ipf-1 in duct cells differed from those in endocrine cells in terms of gel mobility, sequence specificity, and affinity. The observed similarities in Ipf-1 expression by adult human pancreatic duct cells and endocrine cells may reflect their common ontogenic origin, whereas the differences in Ipf-1 phosphorylation and complex formation may correlate with their divergent differentiation.
TYPE OF PUBLICATION: Original article
Articles citing this article:
- Hao E, Tyrberg B, Itkin-Ansari P, Lakey JR, Geron I, Monosov EZ, Barcova M, Mercola M, Levine F.
Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas.
Nat Med
2006.
12: 310-316.
» Diabetes OD » Regeneration of Islets » Stem Cells » Other Cell Sources » Progenitors » Pancreatic Epithelial Cells » Journal Article
- Street CN, Lakey JR, Rajotte RV, Shapiro AM, Kieffer TJ, Lyon JG, Kin T, Korbutt GS.
Enriched Human Pancreatic Ductal Cultures Obtained from Selective Death of Acinar Cells Express Pancreatic and Duodenal Homeobox Gene-1 Age-Dependently.
Rev Diabetic Stud
2004.
1: 66-79.
» Diabetes OD » Regeneration of Islets » Stem Cells » Adult Stem Cells » Transcription Factors » PDX-1 » Journal Article
|
Respond
on this Journal Article!
Hint: Your Response should directly apply to Adult human pancreatic duct and islet cells exhibit similarities in expression and differences in phosphorylation and complex formation of the homeodomain protein IPF-1..
Please check, if this context applies best to your contribution. Otherwise click HERE to change to the appropriate
subject area. The actual subject area is PDX-1.
|
|
|
|
|